GSK3β (phospho Ser9) Polyclonal Antibody

    • Catalog No.:YP0124
    • Applications:IF;WB;IHC;IP;ELISA
    • Reactivity:Human;Mouse;Rat;Drosophila
      • Target:
      • GSK3β
      • Fields:
      • >>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>Chemokine signaling pathway;>>Cell cycle;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Wnt signaling pathway;>>Hedgehog signaling pathway;>>Axon guidance;>>Hippo signaling pathway;>>Focal adhesion;>>Signaling pathways regulating pluripotency of stem cells;>>IL-17 signaling pathway;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>Neurotrophin signaling pathway;>>Dopaminergic synapse;>>Insulin signaling pathway;>>Melanogenesis;>>Prolactin signaling pathway;>>Thyroid hormone signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Cushing syndrome;>>Growth hormone synthesis, secretion and action;>>Alcoholic liver disease;>>Alzheimer disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Yersinia infection;>>Hepatitis C;>>Measles;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpes
      • Gene Name:
      • GSK3B
      • Protein Name:
      • Glycogen synthase kinase-3 beta
      • Human Gene Id:
      • 2932
      • Human Swiss Prot No:
      • P49841
      • Mouse Swiss Prot No:
      • Q9WV60
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9. AA range:1-50
      • Specificity:
      • Phospho-GSK3β (S9) Polyclonal Antibody detects endogenous levels of GSK3β protein only when phosphorylated at S9.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • GSK3B;Glycogen synthase kinase-3 beta;GSK-3 beta;Serine/threonine-protein kinase GSK3B
      • Observed Band(KD):
      • 48kD
      • Background:
      • The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],
      • Function:
      • catalytic activity:ATP + [tau protein] = ADP + [tau protein] phosphate.,enzyme regulation:Inhibited when phosphorylated by AKT1.,function:Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin.,PTM:Phosphorylated by AKT1 and ILK1.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer (By similarity). Interacts with CABYR, MUC1, NIN and PRUNE.,tissue specificity:Expressed in testis, thymus, prostate
      • Subcellular Location:
      • Cytoplasm . Nucleus . Cell membrane . The phosphorylated form shows localization to cytoplasm and cell membrane (PubMed:20937854). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854). .
      • Expression:
      • Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.

      Inhibitor GSK690693 extends Drosophila lifespan via reduce AKT signaling pathway Mech Ageing Dev. 2022 Mar;202:111633. WB Drosophila

      Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis. NATURE STRUCTURAL & MOLECULAR BIOLOGY 2016 Apr 11 WB Human gastric cancer tissues

      Ponicidin inhibits pro-inflammatory cytokine TNF-α-induced epithelial–mesenchymal transition and metastasis of colorectal cancer cells via suppressing the AKT/GSK-3β/Snail pathway. INFLAMMOPHARMACOLOGY Inflammopharmacology. 2019 Jun;27(3):627-638 WB Human 1:1500 HCT116 cell

      Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo. Molecular Medicine Reports Mol Med Rep. 2016 Jan;13(1):720-730 WB Mouse ESCs

      Gastrointestinal Factor GDDR Attenuates Epithelial–Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal. DIGESTIVE DISEASES AND SCIENCES Digest Dis Sci. 2016 Jul;61(7):1941-1949 WB Human gastric cancer tissues SGC7901 cell

      HIV p17 enhances T cell proliferation by suppressing autophagy through the p17‐OLA1‐GSK3β axis under nutrient starvation. JOURNAL OF MEDICAL VIROLOGY J Med Virol. 2021 Jun;93(6):3607-3620 WB Human Jurkat cell

      Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2017; 23: 207–215 WB Rat 1: 1000 hippocampus

      Ou, Liping, et al. "Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo." Molecular medicine reports 13.1 (2016): 720-730.

      Lu, Jian, et al. "Role of LKB1 in migration and invasion of Cr (VI)-transformed human bronchial epithelial Beas-2B cells." Anti-cancer drugs 29.7 (2018): 660-673.

      • Products Images
      • Ma, Xinqi, et al. "METTL3 attenuates proliferative vitreoretinopathy and epithelial‐mesenchymal transition of retinal pigment epithelial cells via wnt/β‐catenin pathway." Journal of Cellular and Molecular Medicine 25.9 (2021): 4220-4234.
      • Ge, Lu, et al. "PRMT5 promotes epithelial‐mesenchymal transition via EGFR‐β‐catenin axis in pancreatic cancer cells." Journal of cellular and molecular medicine 24.2 (2020): 1969-1979.
      • Ou, Liping, et al. "Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo." Molecular medicine reports 13.1 (2016): 720-730.
      • Immunofluorescence analysis of rat-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of rat-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,GSK3β (phospho Ser9) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western Blot analysis of various cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of Hela cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 beta (Phospho-Ser9) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HeLa cells treated with EGF, using GSK3 beta (Phospho-Ser9) Antibody. The lane on the right is blocked with the phospho peptide.