NHRF1 Polyclonal Antibody

    • Catalog No.:YN2924
    • Applications:WB;ELISA
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • NHRF1
      • Fields:
      • >>Tight junction;>>Parathyroid hormone synthesis, secretion and action;>>Pathogenic Escherichia coli infection;>>Human papillomavirus infection
      • Gene Name:
      • SLC9A3R1 NHERF NHERF1
      • Protein Name:
      • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF-1) (Ezrin-radixin-moesin-binding phosphoprotein 50) (EBP50) (Regulatory cofactor of Na(+)/H(+) exchanger) (Sodium-hydrogen exchanger regulatory f
      • Human Gene Id:
      • 9368
      • Human Swiss Prot No:
      • O14745
      • Mouse Swiss Prot No:
      • P70441
      • Immunogen:
      • Synthesized peptide derived from part region of human protein
      • Specificity:
      • NHRF1 Polyclonal Antibody detects endogenous levels of protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000 ELISA 1:5000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Observed Band(KD):
      • 39kD
      • Background:
      • This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009],
      • Function:
      • disease:Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).,function:Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli.,induction:By estrogen.,PTM:Phosphory
      • Subcellular Location:
      • Cytoplasm . Apical cell membrane . Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells. .
      • Expression:
      • Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
      • Products Images
      • Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night