P53 rabbit mAb(ABT239R)

    • Catalog No.:YM6766
    • Applications:WB,IHC-p,IF(paraffin section)
    • Reactivity:Human
      • Gene Name:
      • TP53
      • Protein Name:
      • Cellular tumor antigen p53
      • Human Gene Id:
      • 7157
      • Human Swiss Prot No:
      • P04637
      • Mouse Swiss Prot No:
      • P02340
      • Immunogen:
      • The antibody was produced against synthesized peptide derived from human p53.
      • Specificity:
      • p53 Polyclonal Antibody detects endogenous levels of p53 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit/ IgG1, Kappa
      • Dilution:
      • IF(paraffin section) 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/500. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from Cell culture supernatan by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
      • MolecularWeight(Da):
      • 43653
      • Observed Band(KD):
      • 53
      • Background:
      • tumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013],
      • Function:
      • cofactor:Binds 1 zinc ion per subunit.,disease:Defects in TP53 are a cause of choroid plexus papilloma [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.,disease:Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years a
      • Subcellular Location:
      • nuclear chromatin,nucleus,nucleoplasm,replication fork,transcription factor TFIID complex,nucleolus,cytoplasm,mitochondrion,mitochondrial matrix,endoplasmic reticulum,cytosol,integral component of membrane,nuclear matrix,
      • Expression:
      • Blood,Brain,Classical Hodgkin Lymphoma,Esophageal squamous cell carcinoma,Glial cell,Glial
      • Products Images
      • Western blot analysis of lysates from Lane1: P53 knockdown A431 whole cell lysate, 20ug; Lane2: Scramble mimics knockdown A431 whole cell lysate, 20ug; Lane3: Wild type A431 whole cell lysate, 20ug; primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS0002)was diluted at 1:10000, 37° 1hour. ) GAPDH mAb (cat:YM3029) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS0001)was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-Colon-carcinoma tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, TRIS-EDTA of pH9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human Ovarian-serous-carcinoma tissue. 1,p53 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, TRIS-EDTA of pH9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).