ZAP-70(PT2241) mouse mAb

    • Catalog No.:YM6657
    • Applications:IHC-p,WB
    • Reactivity:Human
    • Description
    • Gene Name:
    • ZAP70 SRK
    • Protein Name:
    • Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-chain associated protein) (Syk-related tyrosine kinase)
    • Human Gene Id:
    • 7535
    • Human Swiss Prot No:
    • P43403
    • Immunogen:
    • Synthesized peptide derived from human ZAP-70
    • Specificity:
    • This antibody detects endogenous levels of human Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-chain associated protein) (Syk-related tyrosine kinase)
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Mouse/IgG1, Kappa
    • Dilution:
    • IHC-p 1:100-500,WB 1:500-2000
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Background:
    • zeta chain of T cell receptor associated protein kinase 70(ZAP70) Homo sapiens This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
    • Function:
    • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.,domain:The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.,function:Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.,online information:ZAP70 mutation db,PTM:Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.,similarity:Contains 1 prote
    • Subcellular Location:
    • Cytoplasmic, Nuclear
    • Expression:
    • Blood,Brain,Leukocyte,Lymphoid,T-cell,
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma. 1,ZAP-70 Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval
    • Immunohistochemical analysis of paraffin-embedded human Tonsil. 1,ZAP-70 Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval
    • Immunohistochemical analysis of paraffin-embedded human Tonsil. 1,ZAP-70 Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval