PGP 9.5 (PT2098) mouse mAb Ready to use

  • Catalog No.:YM6646R
  • Applications:IHC-p,IF(paraffin section)
  • Reactivity:Human
    • Gene Name:
    • UCHL1
    • Protein Name:
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) (EC 3.4.19.12) (EC 6.-.-.-) (Neuron cytoplasmic protein 9.5) (PGP 9.5) (PGP9.5) (Ubiquitin thioesterase L1)
    • Human Gene Id:
    • 7345
    • Human Swiss Prot No:
    • P09936
    • Immunogen:
    • Synthesized peptide derived from human PGP 9.5
    • Specificity:
    • This antibody detects endogenous levels of human PGP 9.5. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
    • Formulation:
    • Liquid in PBS containing, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Mouse/IgG1, Kappa
    • Dilution:
    • IF(paraffin section) 1:50-200 Ready to use for IHC-p, WB application may demands at least 1:5-20 dilution.
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Storage Stability:
    • 4°C/ 1 years
    • Background:
    • ubiquitin C-terminal hydrolase L1(UCHL1) Homo sapiens The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009],
    • Function:
    • catalytic activity:Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).,disease:Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.,function:Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin
    • Subcellular Location:
    • Cytoplasmic
    • Expression:
    • Brain,Cajal-Retzius cell,Fetal brain cortex,Lung,Muscle,