Carcinoembryonic antigen (ABT-CEA) Mouse mAb
- Catalog No.:YM6026
- Applications:IHC;WB;ELISA
- Reactivity:Human
- Target:
- CEA
- Gene Name:
- CEACAM5 CEA
- Protein Name:
- Carcinoembryonic antigen-related cell adhesion molecule 5 (Carcinoembryonic antigen) (CEA) (Meconium antigen 100) (CD antigen CD66e)
- Human Gene Id:
- 1048
- Human Swiss Prot No:
- P06731
- Immunogen:
- Synthesized peptide derived from human carcinoembryonic antigen(CEA) AA range: 600-702
- Specificity:
- This antibody detects endogenous levels of human carcinoembryonic antigen(CEA). Antibody is negative with no cross reaction to BGP or NCA. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Mouse, Monoclonal/IgG2b, Kappa
- Dilution:
- IHC-p 1:200-400, WB 1:200-1000, ELISA 1:5000-20000
- Purification:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 77kD
- Background:
- This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015],
- Function:
- function:Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.,PTM:Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.,similarity:Belongs to the immunoglobulin superfamily. CEA family.,similarity:Contains 7 Ig-like (immunoglobulin-like) domains.,subunit:Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.,tissue specificity:Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.,
- Subcellular Location:
- Cytoplasmic
- Expression:
- Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
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- Antibody-FAQs
- Products Images
- Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CEA(ABT-CEA) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Predicted band size: 76kDa Observed band size: 200kDa
- Human colon carcinoma tissue was stained with anti-CEA(ABT-CEA) antibody.
- Human colon carcinoma tissue was stained with anti-CEA(ABT-CEA) antibody.
- Human lung adenocarcinoma tissue was stained with anti-CEA(ABT-CEA) antibody.
- Human pancreatic adenocarcinoma tissue was stained with anti-CEA(ABT-CEA) antibody.