Keap1 (PTR2556) Mouse mAb
- Catalog No.:YM4696
- Applications:WB;ELISA
- Reactivity:Human; Mouse; Dog (predicted: Rat)
- Target:
- Keap1
- Fields:
- >>Ubiquitin mediated proteolysis;>>Parkinson disease;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Hepatocellular carcinoma;>>Fluid shear stress and atherosclerosis
- Gene Name:
- KEAP1 INRF2 KIAA0132 KLHL19
- Protein Name:
- Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) (Kelch-like protein 19)
- Human Gene Id:
- 9817
- Human Swiss Prot No:
- Q14145
- Mouse Gene Id:
- 50868
- Mouse Swiss Prot No:
- Q9Z2X8
- Rat Gene Id:
- 117519
- Rat Swiss Prot No:
- P57790
- Immunogen:
- Synthesized peptide derived from human Keap1 AA range: 400-500
- Specificity:
- This antibody detects endogenous levels of Keap1 at Human, Mouse,Rat
- Formulation:
- PBS, pH7.4, 50% glycerol, 0.03%Proclin 300
- Source:
- Mouse,monoclonal:IgG1,Kappa
- Dilution:
- WB 1:500-2000,ELISA 1:5000-20000
- Purification:
- Protein G
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) (Kelch-like protein 19)
- Observed Band(KD):
- 70kDa
- Background:
- kelch like ECH associated protein 1(KEAP1) Homo sapiens This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],
- Function:
- disease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degra
- Subcellular Location:
- Cytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839). In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972). .
- Expression:
- Broadly expressed, with highest levels in skeletal muscle.
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- Antibody-FAQs
- Products Images
- Whole cell lysates of A431 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Keap1(PTR2556) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Predicted band size: 69kDa Observed band size: 69kDa
- A431 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Keap1(PTR2556)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: anti-Keap 1 antibody at 1ug/ml Lane 2: anti-Keap 1 antibody at 0.5ug/ml Predicted band size: 69kDa Observed band size: 69kDa
- Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Keap1(PTR2556)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Predicted band size: 69kDa Observed band size: 69kDa