Phospho-Akt (S473) mouse Monoclonal Antibody(7F9)

    • Catalog No.:YM3621
    • Applications:WB,IHC-p,IF(paraffin section)
    • Reactivity:Human
      • Gene Name:
      • AKT1
      • Protein Name:
      • AKT1
      • Human Gene Id:
      • 207
      • Human Swiss Prot No:
      • P31749
      • Mouse Swiss Prot No:
      • P31750
      • Immunogen:
      • Synthetic Peptide of Phospho-Akt (S473) at AA range of 410-490
      • Specificity:
      • Phospho-Akt (S473) protein detects endogenous levels of AKT1
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Mouse
      • Dilution:
      • IF(paraffin section) 1:50-200 WB 1:1000-2000, IHC 1:100-200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • AKT1
      • Observed Band(KD):
      • 60
      • Background:
      • AKT serine/threonine kinase 1(AKT1) Homo sapiens The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime.,disease:Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].,disease:Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).,domain:Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.,domain:The AGC-kinase C-terminal mediates interaction with THEM4.,enzyme regulation:Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.,function:Gene
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,mitochondrion,spindle,cytosol,plasma membrane,cell-cell junction,microtubule cytoskeleton,vesicle,ciliary basal body,protein complex,postsynapse,
      • Expression:
      • Epithelium,Eye,Foreskin,Muscle,Ovary,Placenta,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.
      • Western blot analysis of PC3 Cell Lysate using Phospho-Akt Ser473 Mouse mAb diluted at 1:1000.
      • Xiao, Jun, et al. "Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt‑SREBP‑2 signaling pathway to influence lipid metabolism in HepG2 cells." International journal of molecular medicine 43.5 (2019): 2055-2063.