a-actinin Monoclonal Antibody(4A12)

    • Catalog No.:YM3612
    • Applications:IHC-p,IF(paraffin section)
    • Reactivity:Human,Rat,Mouse
      • Gene Name:
      • ACTN1
      • Protein Name:
      • ACTN1
      • Human Gene Id:
      • 87
      • Human Swiss Prot No:
      • P12814
      • Mouse Swiss Prot No:
      • Q7TPR4
      • Immunogen:
      • Recombinant Protein of a-actinin of ACTN1
      • Specificity:
      • a-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Mouse
      • Dilution:
      • IF(paraffin section) 1:50-200 IHC 1:100-200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ACTN1
      • Observed Band(KD):
      • 103
      • Background:
      • actinin alpha 1(ACTN1) Homo sapiens Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
      • Function:
      • function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,
      • Subcellular Location:
      • stress fiber,ruffle,extracellular region,extracellular space,intracellular,cytoplasm,cytosol,actin filament,plasma membrane,brush border,cell-cell junction,fascia adherens,focal adhesion,Z disc,platelet
      • Expression:
      • Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human Breast Carrcinoma Tissue using a-actinin Mouse mAb diluted at 1:200.
      • Immunohistochemical analysis of paraffin-embedded Human Kidney Tissue using a-actinin Mouse mAb diluted at 1:200.