CK17 Monoclonal Antibody(10A1)

    • Catalog No.:YM3081
    • Applications:WB,IHC-p,IF/ICC,IP
    • Reactivity:Human
      • Gene Name:
      • KRT17
      • Protein Name:
      • Keratin type I cytoskeletal 17
      • Human Gene Id:
      • 3872
      • Human Swiss Prot No:
      • Q04695
      • Mouse Swiss Prot No:
      • Q9QWL7
      • Immunogen:
      • Synthetic Peptide of CK17
      • Specificity:
      • The antibody detects CK17 endogenous proteins.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Mouse
      • Dilution:
      • WB: 1:1000 IP:1:200 IF 1:200 IHC 1:50-300
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • KRT17; Keratin, type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17
      • MolecularWeight(Da):
      • 48106
      • Observed Band(KD):
      • 48
      • Background:
      • keratin 17(KRT17) Homo sapiens This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008],
      • Function:
      • disease:Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.,disease:Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.,disease:KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.,function:May play a role in the
      • Subcellular Location:
      • cytoplasm,intermediate filament,extracellular exosome,cell periphery,
      • Expression:
      • Brain,Cervix,Epithelium,Muscle,Tongue,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CK17 Monoclonal Antibody(10A1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunofluorescence analysis of Human-breast tissue. 1,CK17 Monoclonal Antibody(10A1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, diluted at 1:2000.
      • 1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200