CD31 mouse mAb

    • Catalog No.:YM1375
    • Applications:WB;IP
    • Reactivity:Human
      • Target:
      • CD31
      • Fields:
      • >>Cell adhesion molecules;>>Leukocyte transendothelial migration;>>Malaria;>>Fluid shear stress and atherosclerosis
      • Gene Name:
      • pecam1
      • Human Swiss Prot No:
      • P16284
      • Mouse Swiss Prot No:
      • Q08481
      • Immunogen:
      • Purified recombinant human CD31 protein fragments expressed in E.coli.
      • Specificity:
      • This antibody detects endogenous levels of CD31 and does not cross-react with related proteins.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • wb dilution 1:1000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • Adhesion molecule;CD31;CD31 antigen;CD31 EndoCAM;Endocam;FLJ34100;FLJ58394;GPIIA;GPIIA';PECA1;PECA1_HUMAN;Pecam 1;PECAM 1 CD31 EndoCAM;PECAM;PECAM-1;Pecam1;Platelet endothelial cell adhesion molecule;Platelet/endothelial cell adhesion molecule 1;Platelet/endothelial cell adhesion molecule.
      • Observed Band(KD):
      • 130kD
      • Background:
      • The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010],
      • Function:
      • function:This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.,online information:CD31 entry,online information:PECAM-1,online information:The Singapore human mutation and polymorphism database,PTM:Phosphorylated on Ser and Tyr residues after cellular activation.,similarity:Contains 6 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.,
      • Subcellular Location:
      • Cell membrane ; Single-pass type I membrane protein . Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation. .; [Isoform Long]: Cell membrane ; Single-pass type I membrane protein . Membrane raft . Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. .; [Isoform Delta15]: Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
      • Expression:
      • Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleuk
      • Products Images
      • Western blot detection of CD31 in THP-1 cell lysate using CD31 mouse mAb (1:1000 diluted).Predicted band size: 130KDa.Observed band size: 130KDa.
      • Immunoprecipitation analysis of THP-1 cell lysates using CD31 mouse mAb.