eEF2 mouse mAb

    • Catalog No.:YM1295
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human
      • Target:
      • eEF2
      • Fields:
      • >>AMPK signaling pathway;>>Oxytocin signaling pathway
      • Gene Name:
      • eef2
      • Human Gene Id:
      • 1938
      • Human Swiss Prot No:
      • P13639
      • Mouse Swiss Prot No:
      • P58252
      • Immunogen:
      • Purified recombinant human eEF2 protein fragments expressed in E.coli.
      • Specificity:
      • This antibody detects endogenous levels of eEF2 and does not cross-react with related proteins.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • wb 1:5000 icc 1:200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • EEF2;Eef2;EF-2;EF2;EF2_HUMAN;Elongation factor 2;Eukaryotic translation elongation factor 2;Polypeptidyl tRNA translocase;SCA26.
      • Observed Band(KD):
      • 95kD
      • Background:
      • This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],
      • Function:
      • function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,
      • Subcellular Location:
      • Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. .
      • Expression:
      • Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph
      • Products Images
      • Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.
      • Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using anti-eEF2 mouse mAb (dilution 1:200).