EF-2 Monoclonal Antibody

    • Catalog No.:YM1032
    • Applications:WB
    • Reactivity:Human;Mouse;Rat
      • Target:
      • eEF2
      • Fields:
      • >>AMPK signaling pathway;>>Oxytocin signaling pathway
      • Gene Name:
      • EEF2
      • Protein Name:
      • Elongation factor 2
      • Human Gene Id:
      • 1938
      • Human Swiss Prot No:
      • P13639
      • Mouse Swiss Prot No:
      • P58252
      • Immunogen:
      • Purified recombinant human EF-2 protein fragments expressed in E.coli.
      • Specificity:
      • EF-2 Monoclonal Antibody detects endogenous levels of EF-2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:1000 - 1:2000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • EEF2;EF2;Elongation factor 2;EF-2
      • Molecular Weight(Da):
      • 95kD
      • Background:
      • This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],
      • Function:
      • function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,
      • Subcellular Location:
      • Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. .
      • Expression:
      • Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph
      • Products Images
      • Western Blot analysis using EF-2 Monoclonal Antibody against HL-60 cell lysate.