Synaptotagmin 1 Monoclonal Antibody

    • Catalog No.:YM0605
    • Applications:WB;ELISA
    • Reactivity:Human
      • Target:
      • Synaptotagmin 1/2
      • Fields:
      • >>Synaptic vesicle cycle
      • Gene Name:
      • SYT1
      • Protein Name:
      • Synaptotagmin-1
      • Human Gene Id:
      • 6857
      • Human Swiss Prot No:
      • P21579
      • Mouse Swiss Prot No:
      • P46096
      • Immunogen:
      • Purified recombinant fragment of Synaptotagmin 1 expressed in E. Coli.
      • Specificity:
      • Synaptotagmin 1 Monoclonal Antibody detects endogenous levels of Synaptotagmin 1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • SYT1;SVP65;SYT;Synaptotagmin-1;Synaptotagmin I;SytI;p65
      • References:
      • 1. Cell. 2005 Sep 23;122(6):957-68.
        2. FASEB J. 2008 Jun;22(6):2045-52.
      • Background:
      • The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
      • Function:
      • cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,domain:The first C2 domain mediates Ca(2+)-dependent phospholipid binding.,domain:The second C2 domain mediates interaction with SV2A and STN2.,function:May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subcellular location:Synaptic vesicles and chromaffin granules.,subunit:H
      • Subcellular Location:
      • Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass membrane protein . Cytoplasm .
      • Expression:
      • Expressed in melanocytes (PubMed:23999003).
      • Products Images
      • Western Blot analysis using Synaptotagmin 1 Monoclonal Antibody against truncated Trx-Synaptotagmin 1 recombinant protein (1).