Smad4 Monoclonal Antibody

    • Catalog No.:YM0583
    • Applications:WB,IHC-p,IF/ICC,FCM,ELISA
    • Reactivity:Human
      • Gene Name:
      • SMAD4
      • Protein Name:
      • Mothers against decapentaplegic homolog 4
      • Human Gene Id:
      • 4089
      • Human Swiss Prot No:
      • Q13485
      • Mouse Swiss Prot No:
      • P97471
      • Immunogen:
      • Purified recombinant fragment of human Smad4 expressed in E. Coli.
      • Specificity:
      • Smad4 Monoclonal Antibody detects endogenous levels of Smad4 protein.
      • Formulation:
      • Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
      • Source:
      • Mouse
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • SMAD4; DPC4; MADH4; Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4
      • References:
      • 1. Am J Hum Genet. 2009 Nov;85(5):628-42.
        2. Mol Endocrinol. 2010 Mar;24(3):540-51.
      • Background:
      • SMAD family member 4(SMAD4) Homo sapiens This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009],
      • Function:
      • disease:Defects in SMAD4 are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers.,disease:Defects in SMAD4 are a cause of juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT) [MIM:175050]. JP/HHT syndrome phenotype consists of the coexistence of juvenile polyposis
      • Subcellular Location:
      • nuclear chromatin,intracellular,nucleus,nucleoplasm,transcription factor complex,cytoplasm,cytosol,activin responsive factor complex,SMAD protein complex,RNA polymerase II transcription factor complex,
      • Expression:
      • Fetal brain,Muscle,Placenta,
      • Products Images
      • Western Blot analysis using Smad4 Monoclonal Antibody against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.
      • Immunohistochemistry analysis of paraffin-embedded lung cancer tissues with DAB staining using Smad4 Monoclonal Antibody.
      • Immunofluorescence analysis of NIH/3T3 cells using Smad4 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
      • Flow cytometric analysis of K562 cells using Smad4 Monoclonal Antibody (green) and negative control (purple).