Rab 25 Monoclonal Antibody

    • Catalog No.:YM0546
    • Applications:WB,IHC-p,IF/ICC,FCM,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • RAB25
      • Protein Name:
      • Ras-related protein Rab-25
      • Human Swiss Prot No:
      • P57735
      • Mouse Swiss Prot No:
      • Q9WTL2
      • Immunogen:
      • Purified recombinant fragment of Rab 25 expressed in E. Coli.
      • Specificity:
      • Rab 25 Monoclonal Antibody detects endogenous levels of Rab 25 protein.
      • Formulation:
      • Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
      • Source:
      • Mouse
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • RAB25; CATX8; Ras-related protein Rab-25; CATX-8
      • References:
      • 1. JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422.
        2. Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146.
      • Background:
      • RAB25, member RAS oncogene family(RAB25) Homo sapiens The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq, Nov 2015],
      • Function:
      • function:May selectively regulate the apical recycling and/or transcytotic pathways.,similarity:Belongs to the small GTPase superfamily. Rab family.,subunit:Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4.,
      • Subcellular Location:
      • cytoplasmic, membrane-bounded vesicle,pseudopodium,pseudopodium membrane,cytoplasmic vesicle,extracellular exosome,
      • Expression:
      • Colon,Ovary,Pancreas,Skin,
      • Products Images
      • Western Blot analysis using Rab 25 Monoclonal Antibody against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
      • Immunohistochemistry analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) with DAB staining using Rab 25 Monoclonal Antibody.
      • Immunofluorescence analysis of A549 cells using Rab 25 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
      • Flow cytometric analysis of NIH/3T3 cells using Rab 25 Monoclonal Antibody (green) and negative control (purple).