Rab 10 Monoclonal Antibody

    • Catalog No.:YM0544
    • Applications:WB;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • Rab 10
      • Fields:
      • >>Endocytosis;>>AMPK signaling pathway
      • Gene Name:
      • RAB10
      • Protein Name:
      • Ras-related protein Rab-10
      • Human Swiss Prot No:
      • P61026
      • Mouse Swiss Prot No:
      • P61027
      • Immunogen:
      • Purified recombinant fragment of human Rab 10 expressed in E. Coli.
      • Specificity:
      • Rab 10 Monoclonal Antibody detects endogenous levels of Rab 10 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • RAB10;Ras-related protein Rab-10
      • Molecular Weight(Da):
      • 23kD
      • References:
      • 1. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6508-12.
        2. J Biol Chem. 2003 Apr 25;278(17):15373-80.
        3. Mol Med. 2010 Jul-Aug;16(7-8):247-53.
      • Background:
      • RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009],
      • Function:
      • function:May be involved in vesicular trafficking and neurotransmitter release.,similarity:Belongs to the small GTPase superfamily. Rab family.,
      • Subcellular Location:
      • Cytoplasmic vesicle membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Recycling endosome membrane . Cytoplasmic vesicle, phagosome membrane . Cytoplasm, cytoskeleton, cilium basal body . Endoplasmic reticulum membrane . Cytoplasm, perinuclear region . Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium when phosphorylated by LRRK2 on Thr-73 (PubMed:20576682, PubMed:30398148). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280). .
      • Expression:
      • Expressed in the hippocampus (PubMed:29562525). Expressed in neutrophils (at protein level) (PubMed:29127255). Expressed in the testis (at protein level) (PubMed:28067790).
      • Products Images
      • Western Blot analysis using Rab 10 Monoclonal Antibody against HeLa (1) and NIH/3T3 (2) cell lysate.
      • Immunofluorescence analysis of LOVO cells using Rab 10 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.