NOS3 Monoclonal Antibody

    • Catalog No.:YM0480
    • Applications:IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human
      • Gene Name:
      • NOS3
      • Protein Name:
      • Nitric oxide synthase, endothelial
      • Human Gene Id:
      • 4846
      • Human Swiss Prot No:
      • P29474
      • Mouse Swiss Prot No:
      • P70313
      • Immunogen:
      • Purified recombinant fragment of human NOS3 expressed in E. Coli.
      • Specificity:
      • NOS3 Monoclonal Antibody detects endogenous levels of NOS3 protein.
      • Formulation:
      • Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
      • Source:
      • Mouse
      • Dilution:
      • IF(paraffin section) 1:50-200 Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
      • References:
      • 1. Nature. 1999 Jun 10;399(6736):601-5.
        2. Oncol Rep. 2004 Nov;12(5):1007-11.
        3. Breast Cancer Res Treat. 2008 May;109(1):181-2.
      • Background:
      • nitric oxide synthase 3(NOS3) Homo sapiens Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
      • Function:
      • catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like
      • Subcellular Location:
      • Golgi membrane,nucleus,cytoplasm,cytosol,cytoskeleton,plasma membrane,caveola,endocytic vesicle membrane,
      • Expression:
      • Artery,Placenta,Platelet,Umbilical vein,
      • Products Images
      • Immunohistochemistry analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues with DAB staining using NOS3 Monoclonal Antibody.