NFκB-p105/p50 Monoclonal Antibody

    • Catalog No.:YM0473
    • Applications:WB;IHC;IF;FCM;ELISA
    • Reactivity:Human
      • Target:
      • NFKB1
      • Fields:
      • >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Cellular senescence;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>A
      • Gene Name:
      • NFKB1
      • Protein Name:
      • Nuclear factor NF-kappa-B p108 subunit
      • Human Gene Id:
      • 4790
      • Human Swiss Prot No:
      • P19838
      • Mouse Swiss Prot No:
      • P25799
      • Immunogen:
      • Purified recombinant fragment of human NFκB-p105/p50 expressed in E. Coli.
      • Specificity:
      • NFκB-p105/p50 Monoclonal Antibody detects endogenous levels of NFκB-p105/p50 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000.. IF 1:50-200
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NFKB1;Nuclear factor NF-kappa-B p105 subunit;DNA-binding factor KBF1;EBP-1;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
      • Molecular Weight(Da):
      • 105kD
      • References:
      • 1. Cytokine. 2010 Feb;49(2):215-20.
        2. Chemotherapy. 2009;55(5):381-5.
      • Background:
      • nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isof
      • Function:
      • domain:Glycine-rich region (GRR) appears to be a critical element in the generation of p50.,domain:The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.,function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Diff
      • Subcellular Location:
      • Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
      • Expression:
      • Muscle,Rectum tumor,Uterus,
      • Products Images
      • Western Blot analysis using NFκB-p105/p50 Monoclonal Antibody against K562 (1), Jurkat (2), A431 (3), HeLa (4), THP-1 (5) and MCF-7 (6) cell lysate.
      • Immunohistochemistry analysis of paraffin-embedded prostate tissues (left) and bladder cancer tissues (right) with DAB staining using NFκB-p105/p50 Monoclonal Antibody.
      • Flow cytometric analysis of MCF-7 cells using NFκB-p105/p50 Monoclonal Antibody (green) and negative control (purple).