Akt3 Monoclonal Antibody

    • Catalog No.:YM0020
    • Applications:WB,ELISA
    • Reactivity:Human
      • Gene Name:
      • AKT3
      • Protein Name:
      • RAC-gamma serine/threonine-protein kinase
      • Human Swiss Prot No:
      • Q9Y243
      • Mouse Swiss Prot No:
      • Q9WUA6
      • Immunogen:
      • Purified recombinant fragment of Akt3 expressed in E. Coli.
      • Specificity:
      • Akt3 Monoclonal Antibody detects endogenous levels of Akt3 protein.
      • Formulation:
      • Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
      • Source:
      • Mouse
      • Dilution:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • AKT3; PKBG; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2
      • References:
      • 1. Rachael M. Easton, Han Cho, Kristin Roovers. Mol. Cell. Biol., Mar 2005; 25: 1869 – 1878
        2. Jill M. Stahl, Arati Sharma, Mitchell Cheung. Cancer Res., Oct 2004; 64: 7002–7010.
      • Background:
      • AKT serine/threonine kinase 3(AKT3) Homo sapiens The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.,enzyme regulation:Two specific sites, one in the kinase domain (Thr-305) and the other in the C-terminal regulatory region (Ser-472), need to be phosphorylated for its full activation.,function:IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.,similarity:Contains 1 AG
      • Subcellular Location:
      • intracellular,nucleoplasm,cytoplasm,Golgi apparatus,plasma membrane,
      • Expression:
      • Brain,Eye,Testis,
      • Products Images
      • Western Blot analysis using Akt3 Monoclonal Antibody against truncated Akt3 recombinant protein (1) and human ovary carcinoma tissue lysate (2).