Actin β Monoclonal Antibody

    • Catalog No.:YM0012
    • Applications:WB,IF/ICC,FCM,ELISA
    • Reactivity:Human,Mouse,Rat,Hamster,Monkey
      • Gene Name:
      • ACTB
      • Protein Name:
      • Actin cytoplasmic 1
      • Human Gene Id:
      • 60
      • Human Swiss Prot No:
      • P60709
      • Mouse Swiss Prot No:
      • P60710
      • Immunogen:
      • Synthetic peptide corresponding to amino-terminal residues of human Actin β, conjugated to KLH.
      • Specificity:
      • Actin β Monoclonal Antibody detects endogenous levels of Actin β protein.
      • Formulation:
      • Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
      • Source:
      • Mouse
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ACTB; Actin; cytoplasmic 1; Beta-actin
      • References:
      • 1. Proteomics. 2005 Oct;5(15):3876-84.
        2. PLoS Med. 2005 Oct;2(10):e263.
        3. Mol Biol Cell. 2005 Nov;16(11):5055-60
        4. Nature. 2005 Oct 20;437(7062):1173-8.
      • Background:
      • actin beta(ACTB) Homo sapiens This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
      • Subcellular Location:
      • nuclear chromatin,extracellular space,nucleoplasm,cytoplasm,cytosol,cytoskeleton,plasma membrane,focal adhesion,membrane,intracellular ribonucleoprotein complex,cortical cytoskeleton,NuA4 histone acetyltransferase complex,cyt
      • Expression:
      • B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
      • Products Images
      • Western Blot analysis using Actin β Monoclonal Antibody against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
      • Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Actin β Monoclonal Antibody (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
      • Flow cytometric analysis of MCF-7 cells using Actin β Monoclonal Antibody (right) and negative control (left).