• Target：
• PARP

• Fields：
• >>Base excision repair;>>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Diabetic cardiomyopathy

• Gene Name：
• PARP1

• Protein Name：
• Poly [ADP-ribose] polymerase 1

• Human Gene Id：
• 142

• Human Swiss Prot No：
• P09874

• Mouse Swiss Prot No：
• P11103

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214

• Specificity：
• Cleaved-PARP-1 (D214) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to D214.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000, IF 1:50-300, IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PARP1;ADPRT;PPOL;Poly [ADP-ribose] polymerase 1;PARP-1;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD(+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1

• Observed Band(KD)：
• 24kD

• Background：
• This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT

• Subcellular Location：
• Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .

• Expression：
• Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,

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Osthole inhibits the PI3K/AKT signaling pathway via activation of PTEN and induces cell cycle arrest and apoptosis in esophageal squamous cell carcinoma. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2018 Jun;102:502 WB Human KYSE150 cell,KYSE410 cell

Crocin induces autophagic apoptosis in hepatocellular carcinoma by inhibiting Akt/mTOR activity. OncoTargets and Therapy Oncotargets Ther. 2018; 11: 2017–2028 WB Human HepG2 cell， HCCLM3 cell

Synergistic antitumor effect of suberoylanilide hydroxamic acid and cisplatin in osteosarcoma cells. Oncology Letters 2018 Jul 27 WB Human 143B cell

Inhibition of store‑operated Ca2+ entry counteracts the apoptosis of nasopharyngeal carcinoma cells induced by sodium butyrate. Oncology Letters Oncol Lett. 2017 Feb;13(2):921-929 WB Human 5–8F cell,6–10B cell

Target Identification-Based Analysis of Mechanism of Betulinic Acid-Induced Cells Apoptosis of Cervical Cancer SiHa: Natural Product Communications Hao Sun WB Human

F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C -Dependent Manner Cancers Wei Huang WB Human HCCLM3 cell,HepG2 cell

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