Cleaved-PARP-1 (G215) Polyclonal Antibody

    • Catalog No.:YC0073
    • Applications:WB,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • PARP1
      • Protein Name:
      • Poly [ADP-ribose] polymerase 1
      • Human Gene Id:
      • 142
      • Human Swiss Prot No:
      • P09874
      • Mouse Swiss Prot No:
      • P11103
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PARP. AA range:196-245
      • Specificity:
      • Cleaved-PARP-1 (G215) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to G215.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1
      • MolecularWeight(Da):
      • 113084
      • Observed Band(KD):
      • 89
      • Background:
      • poly(ADP-ribose) polymerase 1(PARP1) Homo sapiens This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT
      • Subcellular Location:
      • nuclear chromosome, telomeric region,nucleus,nuclear envelope,nucleoplasm,transcription factor complex,nucleolus,mitochondrion,membrane,protein complex,
      • Expression:
      • Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,
      • Products Images
      • Western Blot analysis of various cells using Cleaved-PARP-1 (G215) Polyclonal Antibody diluted at 1:500
      • Western Blot analysis of rat-musle cells using Cleaved-PARP-1 (G215) Polyclonal Antibody diluted at 1:500
      • Western blot analysis of lysates from NIH/3T3 cells, treated with etoposide 25uM 1h, using PARP (Cleaved-Gly215) Antibody. The lane on the right is blocked with the synthesized peptide.