• Gene Name：
• NOS3

• Human Gene Id：
• 4846

• Human Swiss Prot No：
• P29474

• Mouse Swiss Prot No：
• P70313

• Rat Swiss Prot No：
• Q62600

• Storage Stability：
• 2-8°C/6 months

• Other Name：
• Nitric oxide synthase, endothelial (EC 1.14.13.39) (Constitutive NOS) (cNOS) (EC-NOS) (Endothelial NOS) (eNOS) (NOS type III) (NOSIII)

• Detection Method：
• Colorimetric

• Background：
• catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subcellular location:Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.,subunit:Homodimer. Interacts with NOSIP and NOSTRIN.,tissue specificity:Platelets, placenta, liver and kidney.,

• Function：
• response to acid, angiogenesis, ovulation from ovarian follicle, blood vessel development, response to hypoxia,vasculature development, blood vessel remodeling, regulation of systemic arterial blood pressure by hormone,regulation of sodium ion transport, response to molecule of bacterial origin, reproductive developmental process,muscle system process, circulatory system process, vascular process in circulatory system, regulation of systemic arterial blood pressure mediated by a chemical signal, regulation of systemic arterial blood pressure, regulation of systemic arterial blood pressure by endothelin, regulation of nucleotide metabolic process, arginine metabolic process,arginine catabolic process, nitric oxide biosynthetic process, anti-apoptosis, cell motion, mitochondrion organization,cell surface receptor linked signal transduction, gamete generation, female gamete generation, sex

• Subcellular Location：
• Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

• Expression：
• Platelets, placenta, liver and kidney.

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