Centaurin-β1 rabbit pAb

  • 货号:YT7777
  • 应用:WB;ELISA
  • 种属:Human;Rat;Mouse;
    • 靶点:
    • Centaurin-β1
    • 简介:
    • >>Endocytosis
    • 基因名称:
    • ACAP1 CENTB1 KIAA0050
    • 蛋白名称:
    • Centaurin-β1
    • Human Gene Id:
    • 9744
    • Human Swiss Prot No:
    • Q15027
    • Mouse Swiss Prot No:
    • Q8K2H4
    • 免疫原:
    • Synthesized peptide derived from human Centaurin-β1 AA range: 490-570
    • 特异性:
    • This antibody detects endogenous levels of Human Centaurin-β1
    • 组成:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • 来源:
    • Polyclonal, Rabbit,IgG
    • 稀释:
    • WB 1:1000-2000 ELISA 1:5000-20000
    • 纯化工艺:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • 浓度:
    • 1 mg/ml
    • 储存:
    • -15°C to -25°C/1 year(Do not lower than -25°C)
    • 其他名称:
    • Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (Centaurin-beta-1;Cnt-b1)
    • 分子量:
    • 81kD
    • 背景:
    • domain:PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.,enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.,function:GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.,miscellaneous:Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular structures derived from endosomal membranes.,PTM:Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 1 BAR domain.,similarity:Contains 1 PH domain.,similarity:Contains 3 ANK repeats.,subunit:Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1.,tissue specificity:Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.,
    • 功能:
    • domain:PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.,enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.,function:GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.,miscellaneous:Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular struct
    • 细胞定位:
    • Recycling endosome membrane ; Peripheral membrane protein ; Cytoplasmic side .
    • 组织表达:
    • Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.