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Ub Polyclonal Antibody

货号：YT5498
应用：WB;ELISA
种属：Human;Mouse;Rat

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靶点：
Ub

简介：
>>Ribosome;>>Ubiquitin mediated proteolysis;>>Mitophagy - animal;>>Parkinson disease;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Kaposi sarcoma-associated herpesvirus infection;>>Coronavirus disease - COVID-19

基因名称：
UBA52/RPS27A/UBB/UBC

蛋白名称：
Ubiquitin

Human Swiss Prot No：
P62987/P62979/P0CG47/P0CG48

免疫原：
Synthesized peptide derived from the N-terminal region of human Ub.

特异性：
Ub Polyclonal Antibody detects endogenous levels of Ub protein.

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Polyclonal, Rabbit,IgG

稀释：
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

纯化工艺：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

浓度：
1 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
UBB;Polyubiquitin-B;UBC;Polyubiquitin-C;RPS27A;UBA80;UBCEP1;Ubiquitin-40S ribosomal protein S27a;Ubiquitin carboxyl extension protein 80;UBA52;UBCEP2;Ubiquitin-60S ribosomal protein L40;CEP52;Ubiquitin A-52 residue ribosomal protein fusion product 1

实测条带：
80 50kD

背景：
Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008],

功能：
function:Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.,miscellaneous:This ribosomal protein is synthesized as a C-terminal extension protein (CEP) of ubiquitin.,miscellaneous:Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a

细胞定位：
[Ubiquitin]: Cytoplasm . Nucleus .; [60S ribosomal protein L40]: Cytoplasm .

组织表达：
Brain,Epithelium,Fetal brain cortex,Liver,L

Studies of proteasome inhibition and apoptosis induction in triple‐negative breast cancer cells by novel amino acid–polypyridine–copper complex. APPLIED ORGANOMETALLIC CHEMISTRY 2019 Jul 22 WB Human 1:1000 MDA-MB-231 cell, CAL-51 cell
货号：YT5498