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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PDP2 Polyclonal Antibody
- 货号:YT5074
- 应用:WB;ELISA;IHC
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2 mitochondrial
- 免疫原:
- Synthesized peptide derived from PDP2 . at AA range: 70-150
- 特异性:
- PDP2 Polyclonal Antibody detects endogenous levels of PDP2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PDP2;KIAA1348;[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial;PDP 2;Pyruvate dehydrogenase phosphatase catalytic subunit 2;PDPC 2
- 背景:
- This gene is a mitochondrial protein that functions as a phosphatase and is involved in the enzymatic resetting of the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2016],
- 功能:
- catalytic activity:[Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H(2)O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.,cofactor:Binds 2 magnesium ions per subunit.,function:Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.,similarity:Belongs to the PP2C family.,subunit:Heterodimer of a catalytic subunit and a FAD protein of unknown function.,
- 细胞定位:
- Mitochondrion matrix .
- Western Blot analysis of extracts from K562 cells, using PDP2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).