β-1,4-Gal-T2 Polyclonal Antibody
- 货号：YT5008
- 应用：IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Galactose metabolism;>>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Other types of O-glycan biosynthesis;>>Mannose type O-glycan biosynthesis;>>Glycosaminoglycan biosynthesis - keratan sulfate;>>Glycosphingolipid biosynthesis - lacto and neolacto series;>>Metabolic pathways
- 蛋白名称：
- Beta-1,4-galactosyltransferase 2
- 免疫原：
- Synthesized peptide derived from the C-terminal region of human β-1,4-Gal-T2.
- 特异性：
- β-1,4-Gal-T2 Polyclonal Antibody detects endogenous levels of β-1,4-Gal-T2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- B4GALT2;Beta-1;4-galactosyltransferase 2;Beta-1,4-GalTase 2;Beta4Gal-T2;b4Gal-T2;UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
- 背景：
- This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity i
- 功能：
- catalytic activity:UDP-galactose + D-glucose = UDP + lactose.,catalytic activity:UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.,catalytic activity:UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.,cofactor:Manganese.,function:Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.,online information:Beta-1,4-galactosyltransferase 2,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 7 family.,subcellular location:Trans cisternae of Golgi stack.,tissue specificity:Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.,
- 细胞定位：
- Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Trans cisternae of Golgi stack.
- 组织表达：
- Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.
- Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
