XRCC1 Polyclonal Antibody
- 货号：YT4917
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Base excision repair
- 蛋白名称：
- DNA repair protein XRCC1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human XRCC1. AA range:517-566
- 特异性：
- XRCC1 Polyclonal Antibody detects endogenous levels of XRCC1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- XRCC1;DNA repair protein XRCC1;X-ray repair cross-complementing protein 1
- 背景：
- The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],
- 功能：
- function:Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.,polymorphism:Carriers of the polymorphic Gln-399 allele may be at greater risk for tobacco- and age-related DNA damage.,PTM:Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.,PTM:Sumoylated.,similarity:Contains 2 BRCT domains.,subcellular location:Accumulates at sites of DNA damage.,subunit:Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF.,
- 细胞定位：
- Nucleus . Moves from the nucleoli to the global nuclear chromatin upon DNA damage. .
- 组织表达：
- Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).
- Western Blot analysis of various cells using XRCC1 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using XRCC1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using XRCC1 Antibody. The lane on the right is blocked with the synthesized peptide.
