Vav1 Polyclonal Antibody

    • 货号:YT4863
    • 应用:WB,ELISA
    • 种属:Human,Mouse,Rat
      • 基因名称:
      • VAV1
      • 蛋白名称:
      • Proto-oncogene vav
      • Human Gene Id:
      • 7409
      • Human Swiss Prot No:
      • P15498
      • Mouse Swiss Prot No:
      • P27870
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human VAV1. AA range:141-190
      • 特异性:
      • Vav1 Polyclonal Antibody detects endogenous levels of Vav1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit
      • 稀释:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • VAV1; VAV; Proto-oncogene vav
      • 分子量:
      • 98314
      • 实测条带:
      • 98
      • 背景:
      • vav guanine nucleotide exchange factor 1(VAV1) Homo sapiens This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012],
      • 功能:
      • domain:The DH domain is involved in interaction with CCPG1.,function:Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.,miscellaneous:'Vav' stands for the sixth letter of the Hebrew alphabet.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:May interact with CCPG1 (By similarity). Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylati
      • 细胞定位:
      • intracellular,cytosol,plasma membrane,cell-cell junction,
      • 组织表达:
      • Widely expressed in hematopoietic cells but not in other cell types.
      • 产品图片
      • Western Blot analysis of various cells using Vav1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of lysates from Jurkat cells, using VAV1 Antibody. The lane on the right is blocked with the synthesized peptide.