VAMP-1/2/3 Polyclonal Antibody
- 货号：YT4850
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>SNARE interactions in vesicular transport
- 蛋白名称：
- Vesicle-associated membrane protein 1/2/3
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human VAMP-1/2/3. AA range:21-70
- 特异性：
- VAMP-1/2/3 Polyclonal Antibody detects endogenous levels of VAMP-1/2/3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:10000. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- VAMP1;SYB1;Vesicle-associated membrane protein 1;VAMP-1;Synaptobrevin-1;VAMP2;SYB2;Vesicle-associated membrane protein 2;VAMP-2;Synaptobrevin-2;VAMP3;SYB3;Vesicle-associated membrane protein 3;VAMP-3;Cellubrevin;CEB;Synaptob
- 背景：
- Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014],
- 功能：
- function:Involved in the targeting and/or fusion of transport vesicles to their target membrane.,similarity:Belongs to the synaptobrevin family.,similarity:Contains 1 v-SNARE coiled-coil homology domain.,subunit:Interacts with VAPA and VAPB.,tissue specificity:Nervous system, skeletal muscle and adipose tissue.,
- 细胞定位：
- [Isoform 1]: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome .; [Isoform 2]: Cytoplasmic vesicle membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome .; [Isoform 3]: Mitochondrion outer membrane; Single-pass type IV membrane protein .
- 组织表达：
- Nervous system, skeletal muscle and adipose tissue.
- Western Blot analysis of mouse-brain cells using VAMP-1/2/3 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunohistochemistry analysis of VAMP-1/2/3 antibody in paraffin-embedded human lung carcinoma tissue.
