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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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SUDD Polyclonal Antibody
- 货号:YT4466
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse
- 蛋白名称:
- Serine/threonine-protein kinase RIO3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RIOK3. AA range:271-320
- 特异性:
- SUDD Polyclonal Antibody detects endogenous levels of SUDD protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RIOK3;SUDD;Serine/threonine-protein kinase RIO3;RIO kinase 3;sudD homolog
- 背景:
- This gene was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The specific function of this gene has not yet been determined. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,similarity:Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.,similarity:Contains 1 protein kinase domain.,tissue specificity:Widely expressed.,
- Western blot analysis of lysates from HepG2 cells, using RIOK3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using RIOK3 antibody.
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).