SRp20 Polyclonal Antibody
- 货号：YT4417
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Spliceosome;>>Amyotrophic lateral sclerosis;>>Herpes simplex virus 1 infection
- 蛋白名称：
- Serine/arginine-rich splicing factor 3
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human SFRS3. AA range:111-160
- 特异性：
- SRp20 Polyclonal Antibody detects endogenous levels of SRp20 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SRSF3;SFRS3;SRP20;Serine/arginine-rich splicing factor 3;Pre-mRNA-splicing factor SRP20;Splicing factor; arginine/serine-rich 3
- 背景：
- The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010],
- 功能：
- function:May be involved in RNA processing in relation with cellular proliferation and/or maturation.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with CPSF6, RBMY1A1 and SFRS12.,
- 细胞定位：
- Nucleus . Nucleus speckle . Cytoplasm . Recruited to nuclear speckles following interaction with YTHDC1. .
- 组织表达：
- Brain,Epithelium,Placenta,Tongue,
- Western Blot analysis of various cells using SRp20 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of Jurkat cells using SRp20 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of 293T lysis using SRp20 antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of MCF7 cells, using SFRS3 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa and Jurkat cells, using SFRS3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
