SMRTe Polyclonal Antibody
- 货号：YT4346
- 应用：IHC;IF;WB;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Notch signaling pathway;>>Epstein-Barr virus infection
- 蛋白名称：
- Nuclear receptor corepressor 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NCOR2. AA range:511-560
- 特异性：
- SMRTe Polyclonal Antibody detects endogenous levels of SMRTe protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NCOR2;CTG26;Nuclear receptor corepressor 2;N-CoR2;CTG repeat protein 26;SMAP270;Silencing mediator of retinoic acid and thyroid hormone receptor;SMRT;T3 receptor-associating factor;TRAC;Thyroid-; retinoic-acid-receptor-associated
- 背景：
- This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011],
- 功能：
- domain:The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).,domain:The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.,function:Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.,induction:Regulated during cell cycle progression.,sequence caution:Contaminating sequence. Sequence of u
- 组织表达：
- Ubiquitous. High levels of expression are detected in lung, spleen and brain.
- Immunofluorescence analysis of A549 cells, using NCOR2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NCOR2 Antibody. The picture on the right is blocked with the synthesized peptide.
