RhoGEF p115 Polyclonal Antibody

    • 货号:YT4088
    • 应用:WB;ELISA;IHC
    • 种属:Human;Mouse;Rat
      • 靶点:
      • RhoGEF p115
      • 简介:
      • >>Vascular smooth muscle contraction;>>Platelet activation;>>Regulation of actin cytoskeleton;>>Parathyroid hormone synthesis, secretion and action;>>Pathogenic Escherichia coli infection;>>Yersinia infection;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Lipid and atherosclerosis
      • 基因名称:
      • ARHGEF1
      • 蛋白名称:
      • Rho guanine nucleotide exchange factor 1
      • Human Gene Id:
      • 9138
      • Human Swiss Prot No:
      • Q92888
      • Mouse Swiss Prot No:
      • Q61210
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human ARHGEF1. AA range:162-211
      • 特异性:
      • RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ARHGEF1;Rho guanine nucleotide exchange factor 1;115 kDa guanine nucleotide exchange factor;p115-RhoGEF;p115RhoGEF;Sub1.5
      • 实测条带:
      • 105kD
      • 背景:
      • Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],
      • 功能:
      • domain:The DH domain is involved in interaction with CCPG1.,domain:The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.,function:Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12.,PTM:Phosphorylated by PKCA.,sequence caution:Contaminating sequence. Sequence of unknown origin in the N-terminal part.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane by activ
      • 细胞定位:
      • Cytoplasm . Membrane . Translocated to the membrane by activated GNA13 or LPA stimulation.
      • 组织表达:
      • Ubiquitously expressed.
      • 产品图片
      • Western Blot analysis of various cells using RhoGEF p115 Polyclonal Antibody
      • Western blot analysis of the lysates from HepG2 cells using ARHGEF1 antibody.
      • Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).