Rab 20 Polyclonal Antibody
- 货号:YT3922
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Ras-related protein Rab-20
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RAB20. AA range:115-164
- 特异性:
- Rab 20 Polyclonal Antibody detects endogenous levels of Rab 20 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RAB20;Ras-related protein Rab-20
- 背景:
- function:Plays a role in apical endocytosis/recycling.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules.,tissue specificity:Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).,
- 功能:
- function:Plays a role in apical endocytosis/recycling.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules.,tissue specificity:Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).,
- 细胞定位:
- Golgi apparatus . Cytoplasmic vesicle, phagosome . Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). .
- 组织表达:
- Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).
- Western Blot analysis of various cells using Rab 20 Polyclonal Antibody diluted at 1:2000
- Western Blot analysis of A549 cells using Rab 20 Polyclonal Antibody diluted at 1:2000
- Western blot analysis of lysates from HeLa, MCF-7, HUVEC, and A549 cells, using RAB20 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using RAB20 antibody.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).