Rab 20 Polyclonal Antibody

    • 货号:YT3922
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Rat;Mouse;
      • 靶点:
      • Rab 20
      • 基因名称:
      • RAB20
      • 蛋白名称:
      • Ras-related protein Rab-20
      • Human Swiss Prot No:
      • Q9NX57
      • Mouse Swiss Prot No:
      • P35295
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human RAB20. AA range:115-164
      • 特异性:
      • Rab 20 Polyclonal Antibody detects endogenous levels of Rab 20 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • RAB20;Ras-related protein Rab-20
      • 实测条带:
      • 28kD
      • 背景:
      • function:Plays a role in apical endocytosis/recycling.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules.,tissue specificity:Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).,
      • 功能:
      • function:Plays a role in apical endocytosis/recycling.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules.,tissue specificity:Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).,
      • 细胞定位:
      • Golgi apparatus . Cytoplasmic vesicle, phagosome . Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). .
      • 组织表达:
      • Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level).
      • 产品图片
      • Western Blot analysis of various cells using Rab 20 Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of A549 cells using Rab 20 Polyclonal Antibody diluted at 1:2000
      • Western blot analysis of lysates from HeLa, MCF-7, HUVEC, and A549 cells, using RAB20 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HeLa cells using RAB20 antibody.
      • Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).