Rab 1B Polyclonal Antibody
- 货号:YT3921
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Ras-related protein Rab-1B
- 免疫原:
- Synthesized peptide derived from Rab 1B . at AA range: 50-130
- 特异性:
- Rab 1B Polyclonal Antibody detects endogenous levels of Rab 1B protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RAB1B;Ras-related protein Rab-1B
- 背景:
- Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009],
- 功能:
- function:Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.,miscellaneous:Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity.,PTM:Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.,subunit:Interacts with MICAL1, MICAL2 and MICAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II.,
- 细胞定位:
- Cytoplasm . Membrane ; Lipid-anchor ; Cytoplasmic side . Preautophagosomal structure membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, perinuclear region . Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments (PubMed:11389151). In the GDP-form, colocalizes with GDI in the cytoplasm (PubMed:11389151). Co-localizes with MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment and to the peri-Golgi region (By similarity). .
- 组织表达:
- B-cell lymphoma,Brain,Muscle,Pancreas,
- Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).