Rab 18 Polyclonal Antibody
- 货号:YT3920
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Ras-related protein Rab-18
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RAB18. AA range:103-152
- 特异性:
- Rab 18 Polyclonal Antibody detects endogenous levels of Rab 18 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RAB18;Ras-related protein Rab-18
- 背景:
- The protein encoded by this gene is a member of a family of Ras-related small GTPases that regulate membrane trafficking in organelles and transport vesicles. Knockdown studies is zebrafish suggest that this protein may have a role in eye and brain development. Mutations in this gene are associated with Warburg micro syndrome type 3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
- 功能:
- function:Plays a role in apical endocytosis/recycling. May be implicated in transport between the plasma membrane and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,tissue specificity:Ubiquitous.,
- 细胞定位:
- Apical cell membrane . Lipid droplet .
- Western Blot analysis of MCF-7 cells using Rab 18 Polyclonal Antibody
- Western blot analysis of lysates from MCF-7 and HUVEC cells, using RAB18 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using RAB18 antibody.
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).