Pitx1 Polyclonal Antibody
- 货号：YT3740
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Pituitary homeobox 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PITX1. AA range:121-170
- 特异性：
- Pitx1 Polyclonal Antibody detects endogenous levels of Pitx1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PITX1;BFT;PTX1;Pituitary homeobox 1;Hindlimb-expressed homeobox protein backfoot;Homeobox protein PITX1;Paired-like homeodomain transcription factor 1
- 背景：
- This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. [provided by RefSeq, Jul 2008],
- 功能：
- function:May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.,similarity:Belongs to the paired homeobox family. Bicoid subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 OAR domain.,
- 组织表达：
- Embryo,Epithelium,Pancreas,Placenta,
- Western Blot analysis of various cells using Pitx1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of Jurkat cells using Pitx1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from Jurkat and COLO cells, using PITX1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
