Oncogene TIM Polyclonal Antibody

    • 货号:YT3461
    • 应用:WB;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Oncogene TIM
      • 基因名称:
      • ARHGEF5
      • 蛋白名称:
      • Rho guanine nucleotide exchange factor 5
      • Human Gene Id:
      • 7984
      • Human Swiss Prot No:
      • Q12774
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human ARHGEF5. AA range:1280-1330
      • 特异性:
      • Oncogene TIM Polyclonal Antibody detects endogenous levels of Oncogene TIM protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ARHGEF5;TIM;Rho guanine nucleotide exchange factor 5;Ephexin-3;Guanine nucleotide regulatory protein TIM;Oncogene TIM;Transforming immortalized mammary oncogene;p60 TIM
      • 实测条带:
      • 60kD
      • 背景:
      • Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008],
      • 功能:
      • similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH3 domain.,tissue specificity:Mainly expressed in kidney, liver, pancreas, lung and placenta.,
      • 细胞定位:
      • Cytoplasm . Nucleus . Cell projection, podosome .
      • 组织表达:
      • Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver (PubMed:15601624). Expressed at low levels in lung and heart (PubMed:14662653).
      • 产品图片
      • Western blot analysis of lysates from RAW264.7 cells, using ARHGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from K562 cells using ARHGEF5 antibody.