NIFK Polyclonal Antibody

    • 货号:YT3127
    • 应用:IHC-p,IF,WB,ELISA
    • 种属:Human,Mouse
      • 基因名称:
      • MKI67IP
      • 蛋白名称:
      • MKI67 FHA domain-interacting nucleolar phosphoprotein
      • Human Swiss Prot No:
      • Q9BYG3
      • Mouse Swiss Prot No:
      • Q91VE6
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human NIFK. AA range:200-249
      • 特异性:
      • NIFK Polyclonal Antibody detects endogenous levels of NIFK protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Rabbit
      • 稀释:
      • WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • MKI67IP; NIFK; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK
      • 分子量:
      • 34222
      • 实测条带:
      • 34
      • 背景:
      • nucleolar protein interacting with the FHA domain of MKI67(NIFK) Homo sapiens This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009],
      • 功能:
      • PTM:Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Localizes to mitotic chromosomes in conjunction with MKI67.,subunit:Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.,
      • 细胞定位:
      • condensed nuclear chromosome,nucleoplasm,nucleolus,cytoplasm,
      • 组织表达:
      • Brain,Cervix carcinoma,Epithelium,Lung,Plac
      • 产品图片
      • Immunofluorescence analysis of HUVEC cells, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.