DNA-PKCS Polyclonal Antibody

    • 货号:YT1385
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • DNA-PKCS
      • 简介:
      • >>Non-homologous end-joining;>>Cell cycle
      • 基因名称:
      • PRKDC
      • 蛋白名称:
      • DNA-dependent protein kinase catalytic subunit
      • Human Gene Id:
      • 5591
      • Human Swiss Prot No:
      • P78527
      • Mouse Swiss Prot No:
      • P97313
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human DNA-PK. AA range:4061-4110
      • 特异性:
      • DNA-PKCS Polyclonal Antibody detects endogenous levels of DNA-PKCS protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • PRKDC;HYRC;HYRC1;DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNA-PKcs;DNPK1;p460
      • 实测条带:
      • 450kD
      • 背景:
      • This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010],
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Inhibited by wortmannin. Activity of the enzyme seems to be attenuated by autophosphorylation.,function:Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of
      • 细胞定位:
      • Nucleus . Nucleus, nucleolus .
      • 组织表达:
      • Brain,Cervix carcinoma,Epithelium,Fetal lung,Placen
      • 产品图片
      • Immunofluorescence analysis of Caco2 cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 488 Secondary antibody (catalog No:RS3211) was diluted at 1:500(room temperature, 50min).
      • Western Blot analysis of various cells using DNA-PKCS Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western Blot analysis of HeLa cells using DNA-PKCS Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DNA-PK Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HeLa cells, using DNA-PK Antibody. The lane on the right is blocked with the synthesized peptide.