Cdc5L Polyclonal Antibody
- 货号：YT0815
- 应用：IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Cell division cycle 5-like protein
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CDC5L. AA range:721-770
- 特异性：
- Cdc5L Polyclonal Antibody detects endogenous levels of Cdc5L protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CDC5L;KIAA0432;PCDC5RP;Cell division cycle 5-like protein;Cdc5-like protein;Pombe cdc5-related protein
- 背景：
- The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008],
- 功能：
- disease:A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.,function:DNA-binding protein involved in cell cycle control. May act as a transcription activator. Also seems to be involved in the second catalytic step of pre-mRNA splicing.,PTM:Phosphorylated; which enhances interaction with PPP1R8.,similarity:Belongs to the CEF1 family.,similarity:Contains 2 HTH myb-type DNA-binding domains.,subcellular location:May shuttle between cytoplasm and nucleus.,subunit:Homodimer. Interacts with DAPK3 (By similarity). Binds to DNA. Binds to adeno-pre-mRNA in an ATP-stimulated manner. Belongs to the spliceosome complex. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Identified in the spliceosome C complex, at least composed
- 细胞定位：
- Nucleus . Nucleus speckle . Cytoplasm . May shuttle between cytoplasm and nucleus. .
- 组织表达：
- Ubiquitously expressed in both fetal and adult tissues.
- Western blot analysis of CDC5L Antibody. The lane on the right is blocked with the CDC5L peptide.
- Immunohistochemistryt analysis of paraffin-embedded human smooth muscle, using CDC5L Antibody. The lane on the right is blocked with the CDC5L peptide.
- Western blot analysis of the lysates from HeLa cells using CDH8 antibody.
