CARD 14 Polyclonal Antibody
- 货号：YT0636
- 应用：IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>NF-kappa B signaling pathway
- 蛋白名称：
- Caspase recruitment domain-containing protein 14
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CAR14. AA range:291-340
- 特异性：
- CARD 14 Polyclonal Antibody detects endogenous levels of CARD 14 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CARD14;CARMA2;Caspase recruitment domain-containing protein 14;CARD-containing MAGUK protein 2;Carma 2
- 背景：
- This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012],
- 功能：
- caution:Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.,function:Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.,similarity:Contains 1 CARD domain.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,subunit:CARD14 and BCL10 bind to each other by CARD-CARD interaction.,tissue specificity:Expressed in placenta. Also detected in HeLa S3 cells, but not in the other cancer cell lines tested.,
- 细胞定位：
- [Isoform 1]: Cytoplasm .; [Isoform 2]: Cytoplasm .; [Isoform 3]: Cytoplasm .
- 组织表达：
- Isoform 1 is detected in placenta and epidermal keratinocytes (PubMed:22521418). Isoform 2 is detected in leukocytes and fetal brain (PubMed:22521418).
- Immunofluorescence analysis of MCF7 cells, using CAR14 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
