Abl1 Polyclonal Antibody

    • 货号:YT0587
    • 应用:WB,ELISA
    • 种属:Human,Mouse,Rat,Monkey
      • 基因名称:
      • ABL1
      • 蛋白名称:
      • Tyrosine-protein kinase ABL1
      • Human Gene Id:
      • 25
      • Human Swiss Prot No:
      • P00519
      • Mouse Swiss Prot No:
      • P00520
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human ABL1. AA range:701-750
      • 特异性:
      • Abl1 Polyclonal Antibody detects endogenous levels of Abl1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit
      • 稀释:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150
      • 分子量:
      • 122873
      • 实测条带:
      • 140(200kd BCR-ABL complex)
      • 背景:
      • ABL proto-oncogene 1, non-receptor tyrosine kinase(ABL1) Homo sapiens This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr
      • 功能:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Magnesium or manganese.,disease:A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).,enzyme regulation:Stabilized in the inactive form by an association between the SH3 domain and the SH2-TK linker region, interactions of the amino-terminal cap, and contributions from an amino-terminal myristoyl group and phospholipids. Activated by autophosphorylation as well as by SRC-family kinase-mediated phosphorylation. Activated by RIN1 binding to the SH2 and SH3 domains. Inhibited by imatinib mesylate (Gleevec) which is used for the treatment of chronic myeloid leukemia (CML).,function:Regulates
      • 细胞定位:
      • ruffle,nucleus,nucleoplasm,nucleolus,cytoplasm,mitochondrion,endoplasmic reticulum,cytosol,actin cytoskeleton,growth cone,extrinsic component of cytoplasmic side of plasma membrane,cell leading ed
      • 组织表达:
      • Brain,Epithelium,Fibroblast,Lung,PCR rescued clones
      • 产品图片
      • Western Blot analysis of cos-7 cells using Abl1 Polyclonal Antibody diluted at 1:2000
      • Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using Abl1 antibody. Antibody was diluted at 1:2000
      • Western blot analysis of lysates from COS7 cells, treated with calyculinA 50ng/ml 30', using ABL1 Antibody. The lane on the right is blocked with the synthesized peptide.