ACTR-IB Polyclonal Antibody

    • 货号:YT0106
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • ACTR-IB
      • 简介:
      • >>Cytokine-cytokine receptor interaction;>>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
      • 基因名称:
      • ACVR1B
      • 蛋白名称:
      • Activin receptor type-1B
      • Human Gene Id:
      • 91
      • Human Swiss Prot No:
      • P36896
      • Mouse Swiss Prot No:
      • Q61271
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human ACV1B. AA range:73-122
      • 特异性:
      • ACTR-IB Polyclonal Antibody detects endogenous levels of ACTR-IB protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ACVR1B;ACVRLK4;ALK4;Activin receptor type-1B;Activin receptor type IB;ACTR-IB;Activin receptor-like kinase 4;ALK-4;Serine/threonine-protein kinase receptor R2;SKR2
      • 实测条带:
      • 56kD
      • 背景:
      • This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010],
      • 功能:
      • catalytic activity:ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.,cofactor:Magnesium or manganese.,function:On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TTRAP.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.,similarity:Contains 1 GS domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with AIP1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Interacts with TTRAP.,tissue specificity:Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.,
      • 细胞定位:
      • Cell membrane ; Single-pass type I membrane protein .
      • 组织表达:
      • Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
      • 产品图片
      • Western Blot analysis of various cells using ACTR-IB Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of HeLa cells, using ACV1B Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 and mouse liver cells, using ACV1B Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).