Actinin-α3 Polyclonal Antibody

    • 货号:YT0103
    • 应用:WB;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Actinin-α3
      • 简介:
      • >>Arrhythmogenic right ventricular cardiomyopathy
      • 基因名称:
      • ACTN3
      • 蛋白名称:
      • Alpha-actinin-3
      • Human Gene Id:
      • 89
      • Human Swiss Prot No:
      • Q08043
      • Mouse Swiss Prot No:
      • O88990
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human ACTN3. AA range:1-50
      • 特异性:
      • Actinin-α3 Polyclonal Antibody detects endogenous levels of Actinin-α3 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ACTN3;Alpha-actinin-3;Alpha-actinin skeletal muscle isoform 3;F-actin cross-linking protein
      • 实测条带:
      • 103kD
      • 背景:
      • This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014],
      • 功能:
      • function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,polymorphism:About 18% of the world population lack a functional ACTN3 due to a stop codon polymorphism at position 577. The absence of a functional ACTN3 expression is not correlated with a disease state.,sequence caution:According to the human genome assembly there is a stop codon in position 577 which is only found in 18% of the world population.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subunit:Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.,tissue specificity:Expressed only in a subset of type 2 skeletal muscle fibers.,
      • 细胞定位:
      • intracellular,cytosol,actin filament,brush border,focal adhesion,Z disc,pseudopodium,extracellular exosome,
      • 组织表达:
      • Expressed only in a subset of type 2 skeletal muscle fibers.
      • 产品图片
      • Western Blot analysis of various cells using Actinin-α3 Polyclonal Antibody
      • Western Blot analysis of HT29 cells using Actinin-α3 Polyclonal Antibody
      • Western blot analysis of lysates from HT-29 cells, using ACTN3 Antibody. The lane on the right is blocked with the synthesized peptide.