Actinin-α1 Polyclonal Antibody
- 货号：YT0100
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Focal adhesion;>>Adherens junction;>>Tight junction;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Shigellosis;>>Amoebiasis;>>Viral carcinogenesis;>>Systemic lupus erythematosus
- 免疫原：
- Synthesized peptide derived from Actinin-α1 . at AA range: 1-80
- 特异性：
- Actinin-α1 Polyclonal Antibody detects endogenous levels of Actinin-α1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ACTN1;Alpha-actinin-1;Alpha-actinin cytoskeletal isoform;F-actin cross-linking protein;Non-muscle alpha-actinin-1
- 背景：
- Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能：
- function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,
- 细胞定位：
- Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cell membrane . Cell junction . Cell projection, ruffle . Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. .
- 组织表达：
- Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue,
- Western blot analysis of 1) 3T3, 2) PC12, 3) Rat Heart Tissue using Actinin-α1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded Mouse KidneyTissue using Actinin-α1 Polyclonal Antibody.
