• 靶点：
• AAK1

• 基因名称：
• AAK1

• 蛋白名称：
• AP2-associated protein kinase 1

• Human Gene Id：
• 22848

• Human Swiss Prot No：
• Q2M2I8

• Mouse Gene Id：
• 269774

• Mouse Swiss Prot No：
• Q3UHJ0

• Rat Swiss Prot No：
• P0C1X8

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320

• 特异性：
• AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• AAK1;KIAA1048;AP2-associated protein kinase 1;Adaptor-associated kinase 1

• 实测条带：
• 94kD

• 背景：
• Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Stimulated by clathrin.,function:Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Active when found in clathrin-coated pits at the plasma membrane.,subunit:Interacts with alpha-adaptin and AP-2.,

• 细胞定位：
• Cell membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse, presynapse . Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. .

• 组织表达：
• Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.

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