Mcl-1 (phospho Ser159) Polyclonal Antibody

    • 货号:YP1190
    • 应用:WB;ELISA;IHC
    • 种属:Human;Mouse
      • 靶点:
      • Mcl-1
      • 简介:
      • >>PI3K-Akt signaling pathway;>>Apoptosis;>>JAK-STAT signaling pathway;>>MicroRNAs in cancer
      • 基因名称:
      • MCL1
      • 蛋白名称:
      • Induced myeloid leukemia cell differentiation protein Mcl-1
      • Human Gene Id:
      • 4170
      • Human Swiss Prot No:
      • Q07820
      • Mouse Swiss Prot No:
      • P97287
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human MCL1 around the phosphorylation site of Ser159. AA range:125-174
      • 特异性:
      • Phospho-Mcl-1 (S159) Polyclonal Antibody detects endogenous levels of Mcl-1 protein only when phosphorylated at S159.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • MCL1;BCL2L3;Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT
      • 实测条带:
      • About 40kd in human,39kd in mouse and rat
      • 背景:
      • This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],
      • 功能:
      • function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the abs
      • 细胞定位:
      • Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.
      • 组织表达:
      • Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th
      • 产品图片
      • Western blot analysis of K562 using p-Mcl-1 (S159) antibody.
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MCL1 (Phospho-Ser159) Antibody
      • Immunofluorescence analysis of A549 cells, using MCL1 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).